碱性磷酸酶ALP

货号：P7070

简介：
Alkaline phosphatase, a cell surface membrane-bound enzyme attached to the cell membrane by means of a phosphatidyl-inositol-glycan anchor, catalyzes the hydrolysis of monophosphate esters at basic pH-releasing inorganic phosphate.
There are four isoenzymes of alkaline phosphatase depending on their tissue site of production. The sites are placental (also termed Regan isoenzyme), intestinal, germ cell, and a tissue nonspecific form produced in the liver, bone, and kidney. However, the major circulating forms in normal individuals are those of bone, liver, and kidney with the bone isoform contributing 40% of the total alkaline phosphatase plasma activity.
The bone and liver isoforms, product of the same genetic loci, exhibit posttranslational modification. Differences in glycosylation between bone and liver isoforms are utilized in chromatographic and lectin binding studies. Additionally, bone form is less stable at high temperature.
碱性磷酸酶（Alkaline Phosphatase，ALP）是一类依赖锌离子、镁离子的金属磷酸单酯水解酶，广泛存在于动物组织，在IVD领域主要分为天然提取ALP与重组ALP，是免疫诊断核心标记酶原料。
ALP单体分子量约50~60 kDa，活性形式多为同源二聚体；酶促反应最适pH区间8.5~10.0，碱性环境下催化磷酸酯底物水解，释放磷酸基团并产生可检测光信号/色原信号