链霉亲和素磁珠性能特点

链霉亲和素磁珠Streptavidin Magnetic Beads，是一种基于链霉亲和素-生物素高亲和力结合原理的磁珠产品，具有高亲和力、高特异性、快速结合、操作简便等核心特点。

一、核心性能
 1. 结合特性：链霉亲和素与生物素结合常数Ka≈10¹⁵ M⁻¹，特异性极强，非特异结合低 。常见结合容量（每mg磁珠）：生物素化抗体≥20–30μg、游离生物素≥1000pmol、25bp生物素化寡核苷酸>500pmol，不同产品有差异 。

The binding constant of streptavidin and biotin is Ka≈10¹⁵ M⁻¹, which is extremely specific and has low non-specific binding. Common binding capacity (per mg magnetic beads): biotinylated antibody ≥ 20–30 μg, free biotin ≥ 1000 pmol, 25bp biotinylated oligonucleotide > 500 pmol, which varies among different products.

 2. 磁学性能：超顺磁性，磁场中快速响应，撤场后均匀分散无残留磁团聚，磁响应快、易分离，适配自动化与高通量。粒径多为50 nm–2.8 µm，比表面积大，动力学高效 。Superparamagnetism, rapid response in the magnetic field, uniform dispersion without residual magnetic agglomeration after the field is removed, fast magnetic response, easy separation, suitable for automation and high throughput. The particle size is mostly 50 nm–2.8 µm, with large specific surface area and efficient kinetics.

3. 稳定性与兼容性：耐受pH 4–9、高盐、变性剂（如尿素）、去污剂，可在多种缓冲液中稳定工作，适合复杂样本处理。

It can withstand high salt, denaturants (such as urea), and detergents. Combined with a wide pH range (pH4-9), it can work stably in a variety of buffers and is suitable for complex sample processing.

 
二、核心特点 
1. 特异性强：只与生物素化分子结合，背景干扰极低，富集纯度高 。

 Only binds biotinylated molecules, with extremely low background interference and high enrichment purity.

2. 操作便捷：磁分离无需离心/过滤，快速高效，减少样品损失，适合微量样本 。

Magnetic separation does not require centrifugation/filtration, is fast and efficient, reduces sample loss, and is suitable for trace samples.

3. 适配性广：兼容蛋白纯化、IP/Co-IP、核酸富集、细胞分选、生物传感器、噬菌体展示等场景 。 

Compatible with protein purification, IP/Co-IP, nucleic acid enrichment, cell sorting, biosensors, phage display and other scenarios

4. 高通量友好：粒径均一、分散性好，适配自动化工作站，支持大规模样品处理。

 It has uniform particle size and good dispersion. It is suitable for automated workstations and supports large-scale sample processing.

5. 共价偶联：链霉亲和素多为共价固定在磁珠表面，减少脱落，提升实验稳定性与重复性 。

Streptavidin is mostly covalently fixed on the surface of magnetic beads to reduce shedding and improve experimental stability and repeatability.